Cisplatin a549

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August undefined, 2024

WebApoptosis of Cisplatin-induced A549 cells was determined by immunofluorescence and flow cytometry, respectively. Western blot was performed to detect protein levels of Prx V, Bcl … WebEffect of MiR-195-5p on the Sensitivity of A549 and A549/DDP Cells to Cisplatin Sensitivity to cisplatin in the different groups was measured as the IC 50 concentration after …

Deferoxamine Counteracts Cisplatin Resistance in A549 Lung ...

WebWe previously had obtained cisplatin-resistant non-small cell lung cancer (NSCLC) A549 cell lines (A549/DDP) and its parental A549 cells. 12,13 In this study, we aim to observe the difference of autophagy between A549/DDP and A549, and also determine the role autophagy plays and the underlying regulation mechanisms during DDP treatment in … WebTargeted therapy for cisplatin‐resistant lung cancer via aptamer‐guided nano‐zinc carriers containing USP14 siRNA Xinmin Zhao,1 ,2 Xianghua Wu,1 ,2 Huijie Wang,1 ,2 Songtao Lai,2 ,3 ,4 and Jialei Wang1 ,2 Xinmin Zhao 1 Department of Thoracic Medical Oncology, Fudan University Shanghai Cancer Center, Shanghai China 2 Department of Oncology, phil mickelson cell phone

Cells exhibit increased fold changes in IC50 concentrat Open-i

WebOct 1, 2024 · As the mechanism of resistance development differs depending on the cancer cell types, we aimed to evaluate the detailed mechanism of resistance to CDDP in two types of lung cancer cells: SBC-3 and A549 cells. The CDDP-resistant SBC-3/DDP and A549/DDP cells were established through continuous treatment with a gradually … WebBottom Line: Characterisation studies demonstrated a decreased proliferative capacity of lung tumour cells in response to cisplatin, increased resistance to cisplatin-induced cell death, accumulation of resistant cells in the G0/G1 phase of the cell cycle and enhanced clonogenic survival ability.The stem cell markers, Nanog, Oct-4 and SOX-2, were … WebJan 20, 2024 · Deferoxamine Counteracts Cisplatin Resistance in A549 Lung Adenocarcinoma Cells by Increasing Vulnerability to Glutamine Deprivation-Induced Cell Death . Authors Wen-Jun Liu 1 2 , Peng-Yu Pan 2 , Ye Sun 3 , Jian-Bo Wang 4 , Huan Zhou 2 , Xin Xie 1 , Zhi-Yuan Duan 1 , Han-Yu Dong 2 , Wen-Na Chen 4 , Li-de Zhang 2 , … tsc watch typescript

Metformin impairs cisplatin resistance effects in A549 lung cancer ...

Targeted therapy for cisplatin‐resistant lung cancer via …

WebFeb 22, 2024 · Lung cancer is the leading cause of cancer-related death in the Unites States, and approximately 85% of all lung cancers are classified as non-small cell lung cancer (NSCLC), which is extremely difficult to treat and its survival rate is low. After decades of clinical trials, the most effective treatments are still those that implement the … Webined if clusterin expression affects the cisplatin sensitivity in vivo; A549, A549/sCLU, and A549/pcDNA3.1 cells were injected subcutaneously into the right flank of nude mice. … phil mickelson cbd oil free sampleWebJan 12, 2024 · To evaluate whether cilastatin influences the antitumor effects of cisplatin in human cancer cells, SCID mice were injected with A549 human lung adenocarcinoma cells. The mice were treated with... phil mickelson cell phone holder

"WebThe IC 50 of cisplatin on A549 cells was 4.07±0.32, 2.26±0.43, and 1.52±0.41 µg/mL, for 24, 48, and 72 hours, respectively. Thus, we used 4, 2, and 1 µg/mL of cisplatin to treat A549 cells... " - Cisplatin a549

Cisplatin a549

Cisplatin and phenanthriplatin modulate long-noncoding RNA …

WebThe A549 cells transfected with sh-PVT1 or sh-NC plasmid were seeded into the right flank of the mice, and 50 μM cisplatin was injected into the intraperitoneal every three days. Consistent with the cellular results, the tumor size, volume, and weight of the sh-PVT1 A549 cell group were significantly lower than the control group (Fig. 3F–H ). WebThe addition of TAS reduced the IC 50 value of cisplatin in A549 and H1299 cells, indicating that TAS can significantly increase the sensitivity of lung cancer cells to cisplatin. Additionally, to determine the effects of the combination of TAS and cisplatin on the apoptosis of NSCLC cells, flow cytometry was used to detect the apoptosis rate ...

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WebNov 1, 2024 · A549 Cells Aged Animals Antineoplastic Agents, Immunological / administration & dosage* Antineoplastic Agents, Immunological / pharmacology B7-H1 Antigen / genetics* B7-H1 Antigen / metabolism* Carcinoma, Non-Small-Cell Lung / drug therapy* Carcinoma, Non-Small-Cell Lung / genetics Carcinoma, Non-Small-Cell Lung / … WebAccording to our former research, asiatic acid (AA) re-sensitized A549/DDP cells to cisplatin (DDP) through decreasing multidrug resistance protein 1 (MDR1) expression level. However, the relevant underlying mechanisms are still unclear. Long non-coding RNA (lncRNA) MALAT1 shows close association with chemo-resistance.

WebSilencing USP14 increased the tumor antagonistic action of DDP in A549 cisplatin‐resistant (A549/DDP) cells, while USP14 overexpression decreased the antagonist effects. … WebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, including lung adenocarcinoma, since it was approved by the Food and Drug …

WebFeb 28, 2024 · All the new complexes showed cytotoxicity in vitro against the three cancer cell lines, but only complex 1a showed statistically significant different activity compared with cisplatin, with significantly lower IC 50 values, 4.31, 6.23, and 4.80 μM, compared with 9.71, 14.48, and 11.59 μM for cisplatin against A549, SKOV3, and MCF-7 ... WebNov 29, 2024 · This study revealed that cisplatin resistant A549 lung cancer cells can be identified by their increased levels of mitochondrial mass. However, Mito-High cells …

WebSilencing USP14 increased the tumor antagonistic action of DDP in A549 cisplatin-resistant (A549/DDP) cells, while USP14 overexpression decreased the antagonist effects. Aptamer-targeted nano-zinc carriers were loaded with USP14 siRNA to …

WebWe used the lung cancer cell line, A549, to determine the effects of the curcuminoids and cisplatin on cell viability and several apoptotic signaling mechanisms. Cellular viability … phil mickelson cell phone holsterWebFeb 28, 2024 · The approximately 85% of lung cancers are non-small cell lung cancer (NSCLC), with dismal median overall survival and 5-year survival rates due to recurrence, extensive invasion and metastasis 2-4. Cisplatin (DDP) is a first-line cytotoxic agent for the treatment of human NSCLC. phil mickelson celebrity net worthWebFeb 28, 2024 · All of these new complexes have been tested on three human cancer cell lines (A549 lung, SKOV3 ovarian, and MCF-7 breast cancer cell lines) and studied for … phil mickelson cbd productsWebNov 29, 2024 · Cisplatin-resistant A549 non-small cell lung cancer cells can be identified by increased mitochondrial mass and are sensitive to pemetrexed treatment Yanyun Gao, … tsc water heater stockWebOur results identified cisplatin resistant subpopulations of NSCLC cells with a putative stem-like signature, providing a further understanding of the cellular events associated with the cisplatin resistance phenotype in lung cancer. tsc washington ncWebAug 15, 2005 · Both BSO and siRNA sensitized melphalan and cisplatin to a small extent . In A549 cells, the GSH level was reduced to 43 ± 2% of the control in the presence of 10 μmol/L of BSO. Therefore, GSH-mediated detoxification seems to play various roles with all the drugs tested in this study, except for l-alanosine. The geldanamycin analogues ... tsc watch don\u0027t clear screenWebThe 50 % inhibitory concentration (IC50) of cisplatin in A549 and A549/DDP cells 48 h after drug treatment Source publication +4 RNA interferences targeting the Fanconi … phil mickelson chipping and pitching videos